Journal: BMC Veterinary Research

Article Title: Chi-miR-3031 regulates beta-casein via the PI3K/AKT-mTOR signaling pathway in goat mammary epithelial cells (GMECs)

doi: 10.1186/s12917-018-1695-6

Figure Lengend Snippet: Increasing chi-miR-3031 and siRNA-IGFBP5 levels notably decreased IGFBP5 mRNA expression. a : Effect of chi-miR-3031 on IGFBP5 mRNA. b : Effect of siRNA-IGFBP5 on IGFBP5 mRNA. P < 0.01 is shown as **. MC: Chi-miR-3031 mimic. NC: Negative control

Article Snippet: Subsequently, the membranes were incubated overnight at 4 °C with a rabbit anti-IGFBP5 polyclonal antibody (1:300, Boster, USA), a rabbit anti-mTOR polyclonal antibody (1:100, Boster, USA), a rabbit anti-p-mTOR polyclonal antibody (1:100, Boster, USA) or a mouse β-actin monoclonal primary antibody (1:1000, Beyotime, Jiangsu, China).

Techniques: Expressing, Negative Control

Journal: BMC Veterinary Research

Article Title: Chi-miR-3031 regulates beta-casein via the PI3K/AKT-mTOR signaling pathway in goat mammary epithelial cells (GMECs)

doi: 10.1186/s12917-018-1695-6

Figure Lengend Snippet: Effects of chi-miR-3031 and siRNA-IGFBP5 on IGFBP5 protein levels. a : Western blot analysis results. b : Densitometric quantification of western blot results. Protein levels were normalized to β-actin. P < 0.05 is shown as *, and P < 0.01 is shown as **. MC: Chi-miR-3031 mimic. NC: Negative control

Article Snippet: Subsequently, the membranes were incubated overnight at 4 °C with a rabbit anti-IGFBP5 polyclonal antibody (1:300, Boster, USA), a rabbit anti-mTOR polyclonal antibody (1:100, Boster, USA), a rabbit anti-p-mTOR polyclonal antibody (1:100, Boster, USA) or a mouse β-actin monoclonal primary antibody (1:1000, Beyotime, Jiangsu, China).

Techniques: Western Blot, Negative Control

Journal: BMC Veterinary Research

Article Title: Chi-miR-3031 regulates beta-casein via the PI3K/AKT-mTOR signaling pathway in goat mammary epithelial cells (GMECs)

doi: 10.1186/s12917-018-1695-6

Figure Lengend Snippet: Protein expression levels of κ-casein ( a ) and β-casein ( b ) in GMECs transfected with MC, NC and siRNA-IGFBP5. P < 0.05 is shown as *, and P < 0.01 is shown as **. MC: Chi-miR-3031 mimic. NC: Negative control

Article Snippet: Subsequently, the membranes were incubated overnight at 4 °C with a rabbit anti-IGFBP5 polyclonal antibody (1:300, Boster, USA), a rabbit anti-mTOR polyclonal antibody (1:100, Boster, USA), a rabbit anti-p-mTOR polyclonal antibody (1:100, Boster, USA) or a mouse β-actin monoclonal primary antibody (1:1000, Beyotime, Jiangsu, China).

Techniques: Expressing, Transfection, Negative Control

Journal: BMC Veterinary Research

Article Title: Chi-miR-3031 regulates beta-casein via the PI3K/AKT-mTOR signaling pathway in goat mammary epithelial cells (GMECs)

doi: 10.1186/s12917-018-1695-6

Figure Lengend Snippet: Effects of chi-miR-3031 and siRNA-IGFBP5 on p-mTOR protein levels. a : Western blot analysis results. b : Densitometric quantification of western blot results. P < 0.05 is shown as *, and P < 0.01 is shown as **. MC: Chi-miR-3031 mimic. NC: Negative control

Article Snippet: Subsequently, the membranes were incubated overnight at 4 °C with a rabbit anti-IGFBP5 polyclonal antibody (1:300, Boster, USA), a rabbit anti-mTOR polyclonal antibody (1:100, Boster, USA), a rabbit anti-p-mTOR polyclonal antibody (1:100, Boster, USA) or a mouse β-actin monoclonal primary antibody (1:1000, Beyotime, Jiangsu, China).

Techniques: Western Blot, Negative Control

Journal: BMC Veterinary Research

Article Title: Chi-miR-3031 regulates beta-casein via the PI3K/AKT-mTOR signaling pathway in goat mammary epithelial cells (GMECs)

doi: 10.1186/s12917-018-1695-6

Figure Lengend Snippet: Primer information for RT-qPCR

Article Snippet: Subsequently, the membranes were incubated overnight at 4 °C with a rabbit anti-IGFBP5 polyclonal antibody (1:300, Boster, USA), a rabbit anti-mTOR polyclonal antibody (1:100, Boster, USA), a rabbit anti-p-mTOR polyclonal antibody (1:100, Boster, USA) or a mouse β-actin monoclonal primary antibody (1:1000, Beyotime, Jiangsu, China).

Techniques: Sequencing

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Expressions of IGFBP-5, cFLIP in cervical intraepithelial neoplasia, cervical carcinoma and their clinical significances: a molecular pathology

doi: 10.1186/1756-9966-28-70

Figure Lengend Snippet: The relationship between expression of IGFBP-5 and cFLIP and clinicopathological parameters in CC

Article Snippet: Primary antibodies used in this study include IGFBP-5 rabbit anti-human polyclonal antibody (Boster Co., Ltd., Wuhan) and cFLIP rabbit anti-human polyclonal antibody (American Neomarker Co.).

Techniques: Expressing

Journal: Experimental Hematology & Oncology

Article Title: Physical activity improves outcomes of combined lenvatinib plus anti-PD-1 therapy in unresectable hepatocellular carcinoma: a retrospective study and mouse model

doi: 10.1186/s40164-022-00275-0

Figure Lengend Snippet: Immunohistochemical staining and analysis of subcutaneous tumors. Immunohistochemical staining was conducted on subcutaneous tumor tissue microarray. H-score were calculated to quantify the expression levels of marker proteins. Representative images captured at 40 ×. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Foxp3 forkhead box protein p3, CTLA4 cytotoxic T-lymphocyte-associated protein 4, TIGIT T cell immunoreceptor with Ig and ITIM domains, TIM3 T-cell immunoglobulin mucin-3, VISTA V domain-containing Ig suppressor of T-cell activation

Article Snippet: Primary Abs used for immunohistochemical staining included: anti-CD4 Ab (#25229, CST), anti-CD8α Ab (#98941,CST), anti-Forkhead box protein p3 (Foxp3) Ab (ab253297, Abcam), anti-F4/80 Ab (#70076, CST), anti-CTLA4 Ab (A00020, Boster), anti-T cell immunoreceptor with Ig and immunoreceptor tyrosine-based inhibitory motif (ITIM) domains (TIGIT) Ab (A01962, Boster), anti-TIM3 Ab (ab241332, Abcam) and, anti-V domain-containing Ig suppressor of T-cell activation (VISTA) Ab (#54979, CST).

Techniques: Immunohistochemical staining, Staining, Microarray, Expressing, Marker, Activation Assay

Journal: Journal of Functional Foods

Article Title: Noni (Morinda citrifolia L.) fruit polysaccharide ameliorated high-fat diet-induced obesity by modulating gut microbiota and improving bile acid metabolism

doi: 10.1016/j.jff.2023.105408

Figure Lengend Snippet: Fig. 2. Effect of NFP on bile acids synthesis in mice under a high-fat diet. (A) The content of TBA in the liver. (B) The expression of CYP7A1 in the liver. (C) Relative protein expression levels of liver FXR. (D) Relative protein expression levels of liver SHP. (E) Bargraph of liver bile acid-related protein expression. (F) Bargraph of intestinal bile acid-related protein expression. (G) Relative protein expression levels of colonic FXR. (H) Relative expression levels of colonic FGF15. (I) Relative expression levels of colonic TGR5. Data are shown as mean ± SEM (n = 5); *p < 0.05, **p < 0.01 versus the NC group; #p < 0.05, ##p < 0.01 versus the HFD group.

Article Snippet: The antibodies GAPDH (BA2913), SHP (A03866-1), and TGR5 (A01958-1) for Western blot detection were purchased from Boster Bioengineering Co., Ltd. (California, USA).

Techniques: Expressing